The ReNature RIO Collection will host key outputs relating to the sustainable use of biodiversity, biodiversity-ecosystem functioning, green infrastructure and ecosystem service assessments across rural-urban gradients, equitable access to the benefits derived from nature in cities and socio-environmental justice, payments for ecosystem services, and designing nature-based solutions. Reports from workshops attended by students, early career researchers and policymakers from all over the world will accumulate further empirical knowledge on the ReNature subjects. This Collection will continue to be developed further as the ReNature project continues to promote open innovation whilst effectively engaging communities in the co-development and exchange of knowledge for nature-based solutions implementation.