The PROMICON RIO Collection presents outcomes from the EU Horizon 2020 project PROMICON (PROductive MIcrobial CONsortia in biotechnology). The project aims to transform biotechnology by applying natural microbiome principles, fostering a shift from monocultures to mixed cultures. PROMICON adopts a dual approach, learning from natural microbiomes (top-down) and creating synthetic microbiomes (bottom-up). The transformative concepts developed by PROMICON are expected to impact the bio-economy sector, with ongoing insights provided through the evolving RIO Collection.