DOI: 10.3897/rio.12.e199260

Authors: Tom Breeze, Riccardo Bommarco, Alexandra-Maria Klein, Ignasi Bartomeus, David Kleijn, Jochen Kantelhardt, Maximilian Koppenberg, Miranda Meuwissen, Henrik Smith, Carla Stoyanova, Mark Brady, Florence Damiens, Teodor Metodiev, Jelena Meinila, Joanna Tusznio, Carlos Ruiz Carreira, Henning Schaak, Sebastian Bekker, Jake Bishop, Shon Ferguson, Klara Fischer, Małgorzata Grodzińska-Jurczak, Hajnalka Szentgyörgyi, Helena Hansson, Romina Rader, René Van Der Wal, Richard Walters, Xiu-shan Li, Zhang Ying, Elena Carp, Simon Potts

Abstract: VALOR is a multi-actor project that will develop a comprehensive systems based approach to develop a deeper understanding of the cascading impacts of pollinator shifts from flower to fork (and beyond). Our work spans seven localised “focal regions” across Europe, where we will explore the impacts of pollinator shifts on ecosystems, farm businesses and local communities through primary research and modelling. These will be supported by broader synthesis and modelling tasks to determine the impacts of pollinators on value chains, international trade and nutrition and highlight the risks to ecosystems and natural capital across Europe, under different co-developed future scenarios. VALOR aims to empower actors to better understand their relationship with pollinators and will produce a number of co-developed tools for landowners businesses and policymakers to better understand these risks and easily undertake their own studies by replicating our methods and employing our models. Throughout the project, VALOR will work through our existing networks of policy and business collaborators to maximise the engagement with our outcomes, datasets and tools, build synergies with the wider pollinator research community and effectively mainstream pollinators into decision-making at all levels throughout Europe.

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DOI: 10.3897/rio.12.e193918

Authors: Erica Zaja, Will Brownlie, Rajabu Hamisi, Maja Arsic, Adelaide Asante, Hilary Barraclough, André Bationo, Martin Blackwell, Dana Cordell, James Elser, Oluwole Fatunbi, Kwame Frimpong, Elise Gallois, Enam Gbekor, Kailyn Harris-Gilliam, Philip Haygarth, Ludwig Hermann, Kenneth Irvine, Issy Lewis, Ning Liu, Vincent Logah, Kaushik Majumdar, Muneta Grace Manzeke-Kangara, Eric Mensah, Malika Mezeli, Michael Miyittah, Innocent Muhwezi, Frederick Otu-Larbi, Richard Padi, Kasper Reitzel, Seth Robinson, Collins Tay, Francis Tetteh, Louis Tetteh, Shamie Zingore, Bryan Spears

Abstract: Phosphorus sustains global food production and is, therefore, of crucial importance to human nutrition and health. However, its mismanagement can lead to water pollution and environmental degradation in addition to low crop yields. In many parts of sub-saharan Africa, phosphorus deficiency constrains agricultural productivity and exacerbates food insecurity. Despite these challenges, phosphorus remains a fragmented topic in global and African policy. To address these issues, the 8th Sustainable Phosphorus Summit (SPS8) was convened in Africa for the first time. SPS8 took place in Accra, Ghana, between 30th September and 3rd October 2025. The Summit was an international collaboration, with co-conveners from the Council for Scientific and Industrial Research (CSIR) – Ghana, the Forum for Agricultural Research in Africa (FARA), The UK Centre for Ecology & Hydrology West Africa Office, Lancaster University and Rothamsted Research. In this paper, we give a detailed overview of the key messages and insights that emerged from highlight talks, lectures, working groups and field trips. We also discuss and reflect on the challenges of delivering an inclusive summit, from designing solutions to benefit-sharing. SPS8 demonstrates that inclusive, cross-sector knowledge-exchange events are crucial to support and enable phosphorus sustainability on the continent of Africa and globally and to enable the next generation of interdisciplinary phosphorus researchers.

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DOI: 10.3897/rio.12.e188665

Authors: Nathaniel Narra, Grischa Fraumann, Raul Palma, Marcin Płóciennik, Michał Błaszczak, Yuansong Qiao

Abstract: The main goal of 'CAgriLab – Consolidated virtual living lab platform for knowledge sharing and adaption in regenerative agriculture' is to establish a consolidated virtual living lab to enable real-time data sharing and collaboration amongst isolated living labs, lighthouses and farmers for exchanging regenerative agriculture knowledge and practices across diverse environments, leveraging Digital Twin (DT), dataspace, blockchain and artificial intelligence (AI) technologies.The CAgriLab consortium is composed of four organisations from three different EU Member States (Ireland, Poland and Finland). It consists of one university of technology, one university of applied sciences, one research centre and one industrial partner. CAgriLab will leverage existing partner projects to pilot and evaluate a decentralised dataspace platform across three countries, covering different use cases i.e. living labs (Ireland), lighthouses (Finland) and farms (Poland). Three sets of DTs (three countries) will be created and connected by the platform.This article contains an edited version of the original funding proposal. The proposal was evaluated by an International Evaluation Committee and selected for funding by the Call Steering Committee.

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DOI: 10.3897/rio.12.e194808

Authors: Nicolae Manta, Cristina Preda, Laurentiu Rozylowicz, Steluta Manolache, Ioana-Minodora Sirbu, Marius Skolka, Cristian Ioja, Iulia Miu, Andreea Nita, Mihai Nita, Iulian Niculae, Mihaela Urziceanu, Eugenia Nagoda, Alina Cislariu, Petronela Camen-Comanescu, Marian Mirea, Lavinia Pindaru, Marius Matache, Paulina Anastasiu

Abstract: Invasive alien species (IAS) represent one of the most significant threats to biodiversity in Romania and across the European Union. Despite the adoption of EU Regulation 1143/2014 on the prevention and management of the introduction and spread of IAS, its effective implementation at national level requires substantial institutional, scientific and operational capacity building. Here, we present a comprehensive national project, COMPLIAS Consolidarea Sistemului National de Management al Speciilor Alogene Invazive din Romania in Conformitate cu Cerintele UE / Strengthening the National Management System for Invasive Alien Species in Romania in Compliance with EU Requirements, SMIS 337191, funded under the Sustainable Development Programme (PDD/216, Priority 2, Specific Objective RSO 2.7) and implemented by the University of Bucharest in partnership with the Ministry of Environment, Waters and Forests of Romania. The project addresses all major obligations under EU Regulation 1143/2014 through seven interconnected work packages: (WP1) updating the regulatory and strategic framework, including the revision of the Invasive Alien Species Action Plan (IAS action plan); (WP2-WP4) comprehensive inventory and mapping of invasive alien species (plants, vertebrates and invertebrates) across Romania; (WP5) developing an early detection and border control system for IAS; (WP6) species distribution modelling, impact assessment and piloting eradication and control techniques for priority IAS; and (WP7) public awareness and stakeholder engagement campaigns. The project will also produce legally binding outputs, including a revised IAS action plan approved by Ministerial Order and mandatory country reports to the European Commission. This paper describes the project's objectives, methodological approach, expected outcomes and its contribution to the EU Biodiversity Strategy for 2030.

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DOI: 10.3897/rio.11.e181727

Authors: Alexandra-Maria Klein, Anda Adamsone-Fiskovica, Georgina Alins, Per Angelstam, Aurelie Belveze, Jordi Bosch, Tom Breeze, Richard Comont, Elise de Groot, Lynn Dicks, Anselm Rodrigo Dominguez, György Dudás, Lotta Fabricius Kristiansen, Mariia Fedoriak, Nicola Gallai, Michael Garratt, Mikelis Grivins, Christina Grozinger, Nigel Jenner, Georgios Kleftodimos, David Kleijn, Anikó Kovács-Hostyánszki, Bodo Krauss, Sara Leonhardt, Julia Osterman, Annie Ouin, Amy Plowman, Simon Potts, Claus Rasmussen, Laura Roquer-Beni, Daniele Rossi, Maj Rundlöf, Oliver Schweiger, Henrik Smith, Jane Stout, Louis Sutter, Martin Thorsøe, George Vlontzos, Dimitry Wintermantel, Nina Kranke, Amibeth Thompson

Abstract: RestPoll is a transdisciplinary project aiming to provide society with tools to reverse wild insect pollinator declines and to position Europe as a global leader in pollinator restoration and set the future agenda for pollinator restoration worldwide. The RestPoll consortium combines the expertise of natural and social scientists, as well as representatives of NGOs, businesses and ministries. RestPoll - together with stakeholders ranging from individual land managers to public authorities - co-designs, evaluates and refines measures and cross-sectoral approaches to restore pollinators and their services. Central to RestPoll is the establishment of a Europe-wide network of pollinator restoration case-study areas with Living Labs, which are unique hubs for experimentation, demonstration and mutual learning at various spatial scales (field, farm, landscape, European scales), in landscapes dominated by intensively managed crops or grasslands. The RestPoll consortium explores, tests, evaluates and refines cross-sectoral pollinator restoration approaches to conserve biodiversity and to benefit nature and society. Our holistic approach also aims to engage in participatory planning and the development of new business models along the food value chain by engaging through newly-developed participatory approaches at diverse social, ecological and political scales. Learning outcomes are communicated to a diverse range of regional and European partners and collaborators, which allows for making a lasting impact beyond the end of the project.

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DOI: 10.3897/rio.11.e160367

Authors: Rajapreethi Rajendran, Claus Weiland, Jonas Grieb, Soulaine Theocharides, Sam Leeflang, Wouter Addink, Sharif Islam

Abstract: The Distributed System for Scientific Collections (DiSSCo) is a research infrastructure to integrate European natural science collections (NSCs) digitally. The aim is to facilitate and enhance the access, management and analysis of collection assets in one unified digital collection. The Machine Annotation Services (MAS) are essential components of DiSSCo’s Digital Specimen Architecture (DSArch). These services automate the annotation of digital objects to enable labelling and categorisation of NSC's digital assets.To further advance this, a Machine Learning as a Service (MLaaS) approach was developed which provides researchers with the access to pre-trained machine-learning models for complex tasks, such as instance segmentation and morphological analysis of datasets. MLaaS enhances the DiSSCo’s scalability and flexibility and allows the integration of machine-learning tools in close alignment with the FAIR (Findable, Accessible, Interoperable, Reusable) principles.This study employs DiSSCO's MLaaS framework for the quantitative analysis of herbarium specimens. Machine-learning models, such as Mask R-CNN and YOLO11, are comparatively applied to detect and generate the pixel-level masks of plant organs in herbarium sheets. Subsequently, these models are used to reconstruct the scale in the herbarium sheet and to calculate the surface area of identified plant organs. The determination of quantitative characteristics of plant specimens, such as measuring leaf area or the timestamp of the floral transition, opens up herbarium data for reuse in the large prognosis platforms currently developed in the framework of the Common European Data Spaces. In this way, plant trait data mobilised from natural science collections can improve the predictive capability of the vegetation model components of climate-related data spaces.

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DOI: 10.3897/arphapreprints.e177889

Authors: Serena Rasconi, Ana Gavrilović

Abstract: Abstracts of talks presented at ParAqua Grantees Conference held in Zagreb (Croatia), 1-2 September 2025

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DOI: 10.3897/arphapreprints.e148125

Authors: Serena Rasconi, Laura Garzoli, Ana Gavrilović

Abstract: Abstracts of talks presented at ParAqua Conference held in Dubrovnik (Croatia), 16-17 April 2024

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DOI: 10.3897/rio.10.e133454

Authors: Yann Hautier, Kathryn Barry, Mariet Hefting, Marijke van Kuijk, Edwin Pos, Betty Verduyn, Rola Johannes, George Kowalchuk, Merel Soons

Abstract: Extreme climate events such as floods and droughts are becoming increasingly frequent and intense across the world. Future climate scenarios predict both an increase in individual extreme events, as well as chronic changes in climatic seasonality. Yet, the combined and relative effects of these pressures on ecosystems remain unknown. Concurrently, human-induced ecological disruption is accelerating species extinction rates, which are estimated to be 100 to 1000 times greater than pre-human levels. This is alarming as greater biological diversity is thought to buffer ecosystem functioning against extreme climate events, thereby safeguarding the provisioning of essential ecological services that contribute to human well-being. However, how and to what extent biodiversity buffers ecosystems against climate variability remains unclear. We recently constructed experimental grassland communities in a mesocosm-based field design representing a realistic gradient of plant diversity. Both extreme events (drought and flood) and a change in seasonality of precipitation are manipulated in a full factorial design to quantify the effects of future seasonal shifts and extremes in precipitation. We will: 1) determine to what extent higher biological diversity ensures that grasslands can continue to provide multiple ecosystem services even in the context of climate change and 2) unravel the fundamental mechanisms by which this is achieved including species asynchrony and positive species interactions. Results of our experimental approach will advance our understanding of the buffering potential of plant diversity and contribute to the development of strategies for sustainable service provisioning of our ecosystems in the face of climate change.

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DOI: 10.3897/rio.10.e131405

Authors: H. Michael G. Lattorff

Abstract: Pollination of crop plants is highly valued as it contributes to productivity in terms of quality and quantity. Globally, pollination is valued at more than USD 500 billion. The primary pollinators are insects and amongst them, bees. The Western honeybee (Apis mellifera) is a very generalist pollinator that is managed. Honeybees contribute up to 50% of the pollination of various crop plants. Pollinators are at risk due to land-use/land-cover changes and agricultural pesticide input.In a recently published study, research on honeybees in runner bean (Phaseolus coccineus L.) in Poland is presented. In one part of the study, the actual foraging of honeybees in beans is recorded, along with the time of day, abundance and time spent on flowers. The second part of the study investigated several apiaries concerning the potential poisoning of bees by means of pesticide exposure. The authors recorded the fitness of colonies, flight activity, behaviour, productivity and pesticide residues in bees.The manuscript, unfortunately, has several scientific flaws that are outlined in this commentary. These flaws, particularly those related to experimental planning and data collection and analysis, have the potential to compromise the conservation of pollinators. The misguidance in the implementation of measures to protect pollinators and pollination services is a cause for concern and should motivate us to address these issues.

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DOI: 10.3897/rio.10.e125192

Authors: Desalegn Chala, Erik Kusch, Claus Weiland, Carrie Andrew, Jonas Grieb, Tuomas Rossi, Tomas Martinovic, Dag Endresen

Abstract: Amidst population growth and climate-driven crop stresses such as drought, extreme weather, fungal and insect pests, as well as various crop diseases, ensuring food security demands innovative strategies. Crop wild relatives (CWR), wild plants in the same genus as the crop as well as wild populations belonging to the same species as the crop, offer novel genetic resources crucial for enhancing crop resilience against these stress factors. Here, we introduce a prototype digital twin (pDT) to aid in searching and utilising CWR genetic resources. Using the MoDGP (Modelling the Germplasm of Interest) tool, the pDT enables mapping geographic areas where stress-tolerant CWR populations can be found. With its graphical user interface, it offers flexibility in selecting genetic resources from CWR tailored to enhance resilience of various crops against diverse stress factors.

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DOI: 10.3897/rio.9.e116070

Authors: Dimitrios Malamataris, Anastasia Tsavdaridou, Dimitra Banti, Athanasios Malliaras, Apostolos Karalis, Nikolaos Theocharis, Christodoulos Michos, Antonios Mazaris

Abstract: Natural resources conservation is considered indispensable for a sustainable future. A thorough managerial analysis of the current and future conservation and availability to meet future demands is both necessary and challenging. As water of adequate quantity and good quality is required for a favourable condition of natural ecosystems and for agricultural production, a comprehensive analysis which would consider hydrological, environmental and agricultural dimensions is needed to properly address their interactions and potential impacts. This study presents a Water-Ecosystems-Food (WEF) nexus methodological flamework aiming at identification and mitigation of critical challenges. The framework is tested in a highly productive water basin in north Greece, the Kokkinorema River Bain, which is also characterised by intense agriculture practices. The presented methodological approach was developed in the context of a natural resources sustainability scheme adapted by the national funded AgroClim project. The selection and prioritisation of the most efficient measures, including Nature-based Solutions, would be driven by a Decision Support System (DSS) tool which will feed upon ecological, social, economic and legislative information. The proposed DSS will also incorporate future climate scenarios to evaluate and address expected future water scarcity, ecosystems degradation and reduced agricultural productivity issues. The proposed methodology for addressing nexus challenges could be transferred to any other natural resources-stressed water basin with similar characteristics.

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DOI: 10.3897/rio.9.e113378

Authors: Helen Hardy, Laurence Livermore, Paul Kersey, Ken Norris, Vincent Smith

Abstract: UK natural science collections hold over 137 million items, an unrivalled source of data about 4.56 billion years of planetary development and hundreds of years of biological change, including the differences made by humans — but the scientific, commercial, and societal benefits of these collections are constrained by the limits of physical access, and by highly fragmented digitisation efforts with less than 10% digitally available. Following work with Frontier Economics in 2021, which showed potential for £2 billion in benefits to the UK economy from digitising all UK natural science collections, in 2022–23 the Natural History Museum London worked, with analytical support from McKinsey and Company, to understand the impact of what has already been digitised and shared by UK natural science collections — what is the demand for these data, what are they used for, and how does this deliver efficient, effective and impactful research?This study focuses on usage via the Global Biodiversity Information Facility, the largest source of relevant usage data, examining 7.6 million records from twelve UK institutions. While these UK collections data are just 0.3% of total GBIF occurrences, they are cited in 12% of peer reviewed publications citing GBIF data, showing the disproportionate impact of UK collections data and the historical, geographical, and taxonomic richness that they bring. Researchers have already benefited from more than £18 million of efficiency savings from digital UK specimen data. Data from natural science collections held in the UK are uniquely impactful resources, vital to a future in which people and planet thrive, and a step change in the pace of digitisation is needed to unlock their potential for researchers, policymakers, and society.

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DOI: 10.3897/arphapreprints.e112382

Authors: Bryony Willcox, Deepa Senapathi, Mark Brown, Simon Potts

Abstract: In the real world, pollinators face multiple interacting pressures, and so response options must be tailored to this. To date, most attention has been on characterising the risks to managed pollinators from single stressors, though recently more attention has been paid to risks from multiple stressors (e.g., pesticides, pathogens and poor nutrition). The PoshBee project has developed a policy brief providing recommendations on options that mitigate against multiple stressors.

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DOI: 10.3897/rio.9.e110501

Authors: Konrad U. Förstner, Anke Becker, Jochen Blom, Peer Bork, Thomas Clavel, Marius Dieckmann, Alexander Goesmann, Barbara Götz, Thomas Gübitz, Franziska Hufsky, Sebastian Jünemann, Marie-Louise Körner, Manja Marz, Ulisses Nunes Da Rocha, Jörg Overmann, Alfred Pühler, Dietrich Rebholz-Schuhmann, Alexander Sczyrba, Jens Stoye, Justine Vandendorpe, Thea Van Rossum, Alice McHardy

Abstract: Microbes – bacteria, archaea, unicellular eukaryotes, and viruses – play an important role in human and environmental health. Growing awareness of this fact has led to a huge increase in microbiological research and applications in a variety of fields. Driven by technological advances that allow high-throughput molecular characterization of microbial species and communities, microbiological research now offers unparalleled opportunities to address current and emerging needs. As well as helping to address global health threats such as antimicrobial resistance and viral pandemics, it also has a key role to play in areas such as agriculture, waste management, water treatment, ecosystems remediation, and the diagnosis, treatment and prevention of various diseases. Reflecting this broad potential, billions of euros have been invested in microbiota research programs worldwide. Though run independently, many of these projects are closely related. However, Germany currently has no infrastructure to connect such projects or even compare their results. Thus, the potential synergy of data and expertise is being squandered. The goal of the NFDI4Microbiota consortium is to serve and connect this broad and heterogeneous research community by elevating the availability and quality of research results through dedicated training, and by facilitating the generation, management, interpretation, sharing, and reuse of microbial data. In doing so, we will also foster interdisciplinary interactions between researchers. NFDI4Microbiota will achieve this by creating a German microbial research network through training and community-building activities, and by creating a cloud-based system that will make the storage, integration and analysis of microbial data, especially omics data, consistent, reproducible, and accessible across all areas of life sciences. In addition to increasing the quality of microbial research in Germany, our training program will support widespread and proper usage of these services. Through this dual emphasis on education and services, NFDI4Microbiota will ensure that microbial research in Germany is synergistic and efficient, and thus excellent. By creating a central resource for German microbial research, NDFDI4Microbiota will establish a connecting hub for all NFDI consortia that work with microbiological data, including GHGA, NFDI4Biodiversity, NFDI4Agri and several others. NFDI4Microbiota will provide non-microbial specialists from these consortia with direct and easy access to the necessary expertise and infrastructure in microbial research in order to facilitate their daily work and enhance their research. The links forged through NFDI4Microbiota will not only increase the synergy between NFDI consortia, but also elevate the overall quality and relevance of microbial research in Germany.

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DOI: 10.3897/arphapreprints.e94590

Authors: Serena Rasconi, Hans-Peter Grossart, Alena Gsell, Bastiaan Willem Ibelings, Dedmer van de Waal, Ramsy Agha, Ariola Bacu, Maija Balode, Meryem Beklioğlu, Maja Berden Zrimec, Florina Botez, Tom Butler, Slawomir Cerbin, Angela Cortina, Michael Cunliffe, Thijs Frenken, Esther Garcés, Laura Gjyli, Yonatan Golan, Tiago Guerra, Ayis Iacovides, Antonio Idà, Maiko Kagami, Veljo Kisand, Jovica Leshoski, Pini Marco, Natasa Mazalica, Takeshi Miki, Maria Iasmina Moza, Sigrid Neuhauser, Deniz Özkundakci, Kristel Panksep, Suzana Patcheva, Branka Pestoric, Maya Petrova Stoyneva, Diogo Pinto, Juergen Polle, Carmen Postolache, Joaquín Pozo Dengra, Albert Reñé, Pavel Rychtecky, Dirk S. Schmeller, Bettina Scholz, Géza Selmeczy, Télesphore Sime-Ngando, Kálmán Tapolczai, Orhideja Tasevska, Ivana Trbojevic, Blagoy Uzunov, Silke Van den Wyngaert, Ellen van Donk, Marieke Vanthoor, Elizabeta Veljanoska Sarafiloska, Susie Wood, Petr Znachor

Abstract: Zoosporic parasites (i.e. fungi and fungi-like aquatic microorganisms) constitute important drivers of natural populations, causing severe host mortality. Economic impacts of parasitic diseases are notable in the microalgae biotech industry, affecting production of food ingredients, biofuels, pharma- and nutraceuticals.While scientific research on this topic is gaining traction by increasing studies elucidating the functional role of zoosporic parasites in natural ecosystems, we are currently lacking integrated and interdisciplinary efforts for effectively detecting and controlling parasites in the microalgae industry. To fill this gap we propose to establish an innovative, dynamic European network connecting scientists, industries and stakeholders to optimize information exchange, equalize access to resources and to develop a joint research agenda. ParAqua aims at compiling and making available all information on the occurrence of zoosporic parasites and their relationship with hosts, elucidate drivers and evaluate impacts of parasitism in natural and man-made aquatic environments. We aim to implement new tools for monitoring and prevention of infections, and to create protocols and a Decision Support Tool for detecting and controlling parasites in the microalgae biotech production. Applied knowledge on zoosporic parasites can feed back from industry to ecology, and we therefore will explore whether the developed tools can be applied for monitoring lakes and reservoirs. Short-Term Scientific Missions and Training Schools will be organised specifically for early stage scientists and managers – with a specific focus on ITC – with the aim to share and integrate both scientific and applied expertise and increase exchange between basic and applied researchers and stakeholders.

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DOI: 10.3897/rio.7.e78844

Authors: Danail Popov, Priyanka Roychoudhury, Helen Hardy, Laurence Livermore, Ken Norris

Abstract: The Natural History Museum, London has been creating digital data about collections for many years, with a formal Digital Collections Programme since 2014. Efforts to monitor the outcomes and impact of this work have focused on metrics of digital access, such as download events, and on citations of digital specimens as a measure of use. Digitisation projects and resulting research have also been used as impact case studies, highlighting areas such as human health and conservation. In 2021, the Museum decided to explore the economic impacts of collections data in more depth, and commissioned Frontier Economics to undertake modelling, resulting in this report. While the methods in this report are relevant to collections globally, this modelling focuses on benefits to the UK, and is intended to support the Museum’s own digitisation work, as well as a current scoping study funded by the Arts & Humanities Research Council about the case for digitising all UK natural science collections as a research infrastructure. This study focuses on digitisation in the round, not distinguishing between different collection types or levels of data creation at this stage. Three methods have been used: first, analysing five key thematic areas or sectors where data from natural science collections are likely to lead to benefits; secondly, analysing typical returns on investment in scientific research; and thirdly, examining the efficiency savings that can be reinvested in research if data are available freely and openly. Together, these methods confirm benefits in excess of £2 billion over 30 years, representing a seven to ten times return on investment.

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DOI: 10.3897/rio.7.e77666

Authors: Judita Tomaskinova, Davide Geneletti, Lynn Dicks, Miriam Grace, Marcus Collier, Davide Longato, Pavel Stoev, Anna Sapundzhieva, Mario Balzan

Abstract: This article presents teaching and learning material from three strategically designed ReNature Horizon 2020 project training activities. A total of twelve presentations covering different aspects of NbS implementation are presented with this article. Additionally, this article also includes materials produced by learners, throughout their work within self-organised groups carried out during the Second ReNature Training School. This is the second article publishing teaching and learning material arising from the ReNature project.

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DOI: 10.3897/rio.6.e56887

Authors: Sugeng Sugiharto, Nursigit Bintoro, Joko Karyadi, Yudi Pranoto

Abstract: The studies of the effect of supercritical carbon dioxide (scCO2) pasteurization on solid food from fish origin are scarcely available. This study was intended to address that gap by investigating the effect of scCO2 on the reduction of muscle protease activity and its impact on physicochemical properties of the Nile tilapia. Tilapia were exposed to CO2 pressure at 70, 75, 80, 85, and 90 bar; temperature at 40 °C; and holding time for 15 min. This study discovered that 80 bar was the minimum pressure to achieve half residual activity of muscle protease and two logs reductions of microbial counts. The applications of 80 and 85 bar were found to achieve significant reduction of tilapia muscle protease activity while still maintained acceptable textural properties. Both 80 and 85 bar were found to be effective to inhibit softening development of tilapia fillet during 14 days of chilled storage. Eighty-five bar and 15 min CO2 pasteurization was considered as maximum level of CO2 pressure that tilapia could withstand without degrading its texture significantly.

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DOI: 10.3897/rio.6.e52052

Authors: Guy Ziv, Michael Beckmann, James Bullock, Anna Cord, Ruth Delzeit, Cristina Domingo, Gunnar Dreßler, Nina Hagemann, Joan Masó, Birgit Müller, Markus Neteler, Anna Sapundzhieva, Pavel Stoev, Jon Stenning, Milica Trajković, Tomáš Václavík

Abstract: Half of the European Union (EU) land and the livelihood of 10 million farmers is threatened by unsustainable land-use intensification, land abandonment and climate change. Policy instruments, including the EU Common Agricultural Policy (CAP) have so far failed to stop this environmental degradation. BESTMAP will: 1) Develop a behavioural theoretical modelling framework to take into account complexity of farmers’ decision-making; 2) Develop, adapt and customize a suite of opensource, flexible, interoperable and customisable computer models linked to existing data e.g. LPIS/IACS and remote sensing e.g. Sentinel-2; 3) Link economic, individual-farm agent-based, biophysical ecosystem services and biodiversity and geostatistical socio-economic models; 4) Produce a simple-to-use dashboard to compare scenarios of Agri-Environmental Schemes adoption; 5) Improve the effectiveness of future EU rural policies’ design, monitoring and implementation.

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DOI: 10.3897/rio.5.e35906

Authors: Sulav Paudel, Istvan Miko, Edwin Rajotte, Gary Felton

Abstract: The Lyonet’s gland is a widespread accessory labial gland in Lepidoptera. Although its function is ambiguous, the Lyonet’s gland arguably plays an important role in silk production. Our knowledge of the Lyonet’s gland in heliothine species is extremely limited; it is reportedly missing from Helicoverpa armigera and Heliothis virescens, whereas it is reportedly reduced in size in Helicoverpa zea. Using confocal microscopy and brightfield imaging, we show that the Lyonet’s gland in Helicoverpa zea is present and the size is relatively enlarged relative to other lepidopterans. We also examined whether glucose oxidase, an abundant enzyme found in labial salivary gland is also present in the extracts of Lyonet’s gland, but we found no evidence of that. Based on the size and accessibility of the Lyonet’s gland, future studies should include transcriptomic and proteomics studies in H. zea to provide evidence for potential functions.

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DOI: 10.3897/rio.2.e8134

Authors: Nik-Othman Abdullah

Abstract:

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