Bi-directional immuno-modulation by Matrix Metalloproteinase-7 (MMP-7) and A Disintegrin And Metalloproteinase-17 (ADAM-17) as transplantation rejection-tolerance spectrum

Theoretically, Matrix Metalloproteinase-7 (MMP-7) leads to allograft rejection, and A Disintegrin and Metalloproteinase-17 (ADAM-17) results in allograft tolerance. The research proposal utilizes the animal model of knock-out mice to perform transplant surgery and then detect or measure allograft rejection by selected serum biomarker and tissue typing. Comparisons will be made for knock-out, wild-type, and wild-type treated with proteinase inhibitors. Methodological and theoretical details will be elucidated and revised as the research goes on.

Immuno-modulator drugs that does not only suppress but also enhance immunity in transplant.
We can quantify post-transplant patients' immunity status and adjust bi-directionally the immunity levels whenever rejection or infection occurs.
Surgery: transplantation of s specified organ, such as kidney, heart, aorta, lungs, etc.
Drafting of immune synapse networks from MMP-7 and ADAM-17.
Measurement of rejection by serum marker and tissue typing; 3.
Repeat the above steps 1 to 3 on mice treated with protease inhibitors; 5.
Repeat the whole experiment for replicability and reproducibility; 7.
Results interpretatiom and report writing.

Work plan
The research will be carried out with the mentorship of Dr. Wei-Hsuan Yu, PhD, Laboratory of Matrix Biology, Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.

1.
Decision of which organ to transplant on mice; 2.
Decision of which serum marker and tissue typing to use; 3.
Repeat the whole experiment for replicability and reproducibility; 5.
Report writing and publication.

Details for replicability and reproducibility
To achieve replicability and reproducibility, we will repeat the same surgery, sample collection, lab analysis, and statistical analyses on at least two individual mice.If time and funding permit, we will repeat the experiment on more mice.
(2) Experimental data obtained from our animal model and degradomics will be compared and explored with the use of MatrixDB (Launay et al. 2015)

Expected results and impact
Expected Results