Corresponding author: Robert Jeenchen Chen (
Academic editor:
Cardiac regeneration may be the future ultimate therapy for terminal heart failure. Nucleostemin (GNL3) plays a pivotal role in cardiac repair. Induced pluripotent stem (iPS) cells, induced by SOX2-OCT4-NANOG complex, may offer clues form regeneration. We would like to explore the interactions by bioinformatic approach. We identified the candidates that interacted with nucleostemin (GNL3), SOX2, OCT4 (POU5F1), and NANOG with NCBI online Entrez Gene and PubMed. The pathway networks were built with Ingenuity Pathway Analysis (IPA) 7.5 ®.
In GNL3 network, molecules related to cardiovascular system development and function included CDKN2A, TP53 (tumor protein p53), ID3, NPM1, and IL2 (p=8.4e-4 ~ 9.9e-4 by right-tailed Fisher Exact Test). In canonical pathways, PPP2R5A involved in cardiac beta-adrenergic signaling and Fgf (fibroblast growth factor) involved in human embryonic stem cell pluripotency. Nucleostemin and iPS networks had two common molecules: Fgf and TP53. Nucleostemin and iPS have interactions via tumor protein P53 and fibroblast growth factor, which would require future research.
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Gold Prize of the Best Presentation of the Young Investigator Award, the Biennial Congress of the Association of Thoracic and Cardiovascular Surgeons of Asia (ATCSA) (
Although there are clinical advances in mechanical circulatory support devices and heart transplantation, the ultimate therapy for a failing heart may be cardiac regeneration from reprogrammed somatic cells or induced pluripotent stem cells, which avoids the pitfalls in ethics of embryonic cells, device anticoagulants, or immunosuppressants (
Literature review was done with NCBI online Entrez Gene and PubMed to identify the candidate molecules that may interact with nucleostemin (GNL3), SOX2, OCT4 (POU5F1), and NANOG (
Functional pathway networks were built for nucleostemin (GNL3), with the interactions with the iPS (SOX2-OCT4-NANOG) network in the subcellular level (Fig.
In the exploratory analysis, the functional pathway networks of nucleostemin and iPS interact via TP53 (tumor protein P53) and Fgf (fibroblast growth factor), which could be further investigated to provide clues for the future research of postnatal cardiac regeneration (
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Nucleostemin (GNL3) and iPS (SOX2-OCT4-NANOG) subcellular interaction network