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Research Ideas and Outcomes 10: e116907
https://doi.org/10.3897/rio.10.e116907 (10 May 2024)
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  • Introduction
    • Research Questions
    • Study aims and Objectives
  • Background and Study Impact
    • Uterine Fibroids (UF)
    • Aetiology and Risk factors
    • Altered Microbiome and Epigenetic regulation
    • Genetic Alterations of UF
    • Therapeutics and Diagnostics
    • Study Impact
  • Implementation
    • Study workflow
    • Study Design
    • Ethical Clearance
    • Sample size determination
    • Sample Collection
    • Assessment of demographic factors and clinical manifestations of UF in Ghanaian women
    • Determining uterine microbial profile from UF cases and controls
      • Sample preparation
      • Shotgun metagenomics sequencing and data analysis
      • Role of patient-derived microbiome on fibroid tumorigenesis
    • Profiling molecular markers associated with UF
      • RT-qPCR
    • Functional analysis of identified marker in-vivo
    • Genotyping the unique marker associated with UF
    • Bioinformatics and Statistical Analyses
    • Expected outcomes
  • Acknowledgements
  • References
  • Supplementary files
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